February 13 @ 11:00 AM – 12:00 PM
Dr. Yongdeng Zhang
Assistant Professor, School of Life Sciences
Westlake University
“Elucidating subcellular architecture and dynamics with super-resolution microscopy”
Abstract: Super-resolution microscopy plays a crucial role in elucidating the intricate molecular mechanisms underlying diverse biological processes. Here, we present the development of a highly efficient multicolor 4Pi-SMS technique that enables the acquisition of 3D super-resolution images of whole mammalian cells with a resolution of approximately 10 nm. When combined with expansion microscopy, molecular-level resolution (<5 nm) can be achieved. Additionally, notable progress has been made in attaining high-quality 3D super-resolution imaging of biological tissue samples. This involves the utilization of multi-line scanning illumination and confocal detection to reduce out-of-focus background, as well as deep-learning-enabled aberration correction with adaptive optics. Furthermore, by combining 4Pi microscopy with 3D structured illumination microscopy, we implemented 4Pi-SIM, which achieved time-lapse volumetric isotropic 100 nm resolution in 3D in living cells. Through imaging a variety of biological samples, we demonstrated that 4Pi-SIM enables the visualization of subcellular structures with excellent clarity and detail. These developments hold great potential for elucidating subcellular architecture and revealing dynamic behaviors to enhance our comprehension of biological processes at the nanoscale level.
Host: Fangyuan Ding
Seminar will be held in person only.
Developmental & Cell Biology Fall 2024 Seminar Series
Each quarter the Department of Developmental and Cell Biology hosts a weekly seminar. The purpose of these seminars is to enable experts from around the country to share their newest discoveries and ideas with our students and faculty. Seminars are held on Thursdays at 11:00 a.m., in Natural Sciences II room 4201.
For questions about this event, please contact Mayra Rubio at mrubio3@uci.edu.